Biopython 1.77 released

Biopython 1.77 has been released and is available from our website and PyPI.

This is the first release since we dropped support for Python 2.7 and 3.5. Focusing on Python 3.6 or later will let us take advantage of new functionality and syntax, and simplify our code base and testing.

This release of Biopython supports Python 3.6, 3.7 and 3.8 It has also been tested on PyPy3.6.1 v7.1.1.

pairwise2 now allows the input of parameters with keywords and returns the alignments as a list of namedtuples.

The codon tables have been updated to NCBI genetic code table version 4.5, which adds Cephalodiscidae mitochondrial as table 33.

Bio.Restriction has been updated to the January 2020 release of REBASE.

A major contribution by Rob Miller to Bio.PDB provides new methods to handle protein structure transformations using dihedral angles (internal coordinates). The new framework supports lossless interconversion between internal and cartesian coordinates, which, among other uses, simplifies the analysis and manipulation of coordinates of protein structures.

As in recent releases, more of our code is now explicitly available under either our original “Biopython License Agreement“, or the very similar but more commonly used “3-Clause BSD License“. See the LICENSE.rst file for more details.

Additionally, a number of small bugs and typos have been fixed with further additions to the test suite. There has been further work to follow the Python PEP8, PEP257 and best practice standard coding style, and more of the code style has been reformatted with the black tool.

Many thanks to the Biopython developers and community for making this release possible, especially the following contributors:

  • Alexander Decurnou (first contribution)
  • Andrei Istrate (first contribution)
  • Andrey Raspopov
  • Austin Varela (first contribution)
  • Chris Daley
  • Chris Rands
  • Deepak Khatri
  • Hielke Walinga (first contribution)
  • Kai Blin
  • Karthikeyan Singaravelan (first contribution)
  • Markus Piotrowski
  • Michiel de Hoon
  • Peter Cock
  • Rob Miller
  • Sergio Valqui
  • Steve Bond
  • Sujan Dulal (first contribution)
  • Tianyi Shi (first contribution)


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