Bioperl

A possible release candidate for BioPerl 1.2.2 is now available for download: http://bioperl.org/DIST/bioperl-1.2.2rc1.tar.gz

Please download and give it a test. New aditions, features and fixes are in the “Changes” file.

Heikki has converted some Bioperl workshop materials to a web slide show that is available here:

http://www.ebi.ac.uk/~lehvasla/bioperl/

Do you need to access sequences from multiple places? Would you like to easily retrieve your own local sequences from indexed flat files, all other sequences on species X from department wide raletional database and the rest from global internet servers?

The Open Biological Database Access (OBDA) System was designed so that one could use the same application code to access data from all three of the database types by simply changing a few lines in a “configuration file”. This makes application code more portable and easier to maintain.

Shawn Hoon announces the release of bioperl-run-1.2.0 which is an extention to the bioperl framework that contains modules that act as wrappers for common informatics applications.

The full release announcement can be read here: http://bioperl.org/pipermail/bioperl-l/2003-May/012308.html

Jonathan Epstein has a simple script and directions for browsing bioperl list traffic offline via a mobile device.

The full post can be read online at: http://bioperl.org/pipermail/bioperl-l/2003-May/012287.html

Tony writes: {{ double-space-with-newline }} I have just checked in to the Bio::Das perl beta CVS repository (“Bio-Das2”) a collection of modules that creates a minimal DAS reference server from a single AGP file (or a directory of one or more files). There is also a sample server script in the “eg” directory.

Briefly, the server is started using something like:

cd ./eg
./agpserver --dsn ncbi31 --port 3000 --agpfile ./AGP/chr1.agp

It can then be used by a DAS client in the normal way. It is simple (no frills!) and capable of serving assembly information, entry_points, DSN info, features across a segment and features by ID. No other DAS commands are supported yet.

Jason writes:

I’ve added a parser for the ab initio gene predictor GlimmerM in Bio::Tools::Glimmer. Tests are added to t/Genpred.t now.

It follows the Bio::Tools::AnalysisParser interface which support the next_prediction call and returns a Bio::Tools::Prediction::Gene object in the same way as Genscan.

I also have some scripts for building the training set file so you can run Glimmer on other species. There is also a similar script for building custom splice sites file for Genewise which I’ll commit soon. Basically it takes as input a GFF file which has exons annotated (typically from Sim4/Exonerate alignments of cDNA to DNA) and a reference to the genomic DNA db (typically just a flatfile which we index with Bio::DB::Flat).

Heikki writes:

Philip Lijnzaad has written a new sequence format module called ’tab’. It is in CVS. Here is the blurb he wrote:

It is very useful when doing large scale stuff using the Unix command line utilities (grep, sort, awk, sed, split, you name it). Imagine that you have a format converter ‘seqconvert’ along the following lines:

my $in  = Bio::SeqIO->newFh(-fh => *STDIN , '-format' => $from);
my $out = Bio::SeqIO->newFh(-fh=> *STDOUT, '-format' => $to);
print $out $_ while <$in>;

then you can very easily filter sequence files for duplicates as:

Nigam Shah has created the necessary PPM/PPD distribution files for bioperl-1.2 .1 that will allow ActiveState perl users on Windows machines to easily install the bioperl suite and the most common bioperl external module dependencies. The PPD file is online at http://bioperl.org/ftp/DIST/Bioperl-1.2.1.ppd. The actual PPD file is at http://bioperl.org/DIST/bioperl-1.2.1-ppm.tar.gz

More information on bioperl Windows installations can be read online at http://www.bioperl.org/Core/Latest/INSTALL.WIN